Behavioural Assessment of Blood-Brain Barrier Opening Induced by Various Ultrasound Parameters

  • Feng-Yi Yang
  • Sheng-Fang Huang
Keywords: behavioural evaluation, focused ultrasound, blood-brain barrier, memory


Focused ultrasound (FUS)-induced opening of the blood-brain barrier (BBB) in the presence of microbubbles is prospective for a targeted drug delivery to the brain lesion. However, the safety of this technology has been addressed recently. The purpose of this study was to assess the behavioural changes following the FUS induced-BBB disruption (BBBD). The behavioural assessment of rats were tested using the open field and hole-board from day 1 through day 32 after undergoing BBBD induced by FUS with either a mild or heavy parameter. We found that heavy BBBD induced behavioral changes, including significantly increased locomotor activity and a longer latency to nose poke for baits. Additionally, mild BBBD resulted in significant decrease of central activity. Therefore, the behavioural changes after FUS induced BBBD should be considered before clinical application.


F. Y. Yang, T. T. Wong, M. C. Teng, R. S. Liu, M. Lu, and H. F. Liang, et al., “Focused ultrasound and interleukin-4 receptor- targeted liposomal doxorubicin for enhanced targeted drug delivery and antitumor effect in glioblastoma multiforme,” Journal of Controlled Release, vol. 160, no. 3, pp. 652-658, June 2012.

F. Y. Yang, W. Y. Chang, J. J. Li, H. E. Wang, J. C. Chen, and C. W. Chang, “Pharmacokinetic analysis and uptake of 18F-FBPA-Fr sfter ultrasound-induced blood-brain barrier disruption for potential enhancement of boron delivery for neutron capture therapy,” Journal of Nuclear Medicine, vol. 55, pp. 616-621, April 2014.

F. Yang, W. Fu, W. Chen, W. Yeh, and W. Lin, “Quantitative evaluation of the use of microbubbles with transcranial focused ultrasound on blood¡Vbrain-barrier disruption,” Ultrasonics Sonochemistry, vol. 15, no. 4, pp. 636-643, April 2008.

F. Y. Yang, W. Y. Chang, J. C. Chen, L. C. Lee, and Y. S. Hung, “Quantitative assessment of cerebral glucose metabolic rates after blood-brain barrier disruption induced by focused ultrasound using FDG-MicroPET,” Neurolmage, vol. 90, pp. 93-98, April 2014.

How to Cite
Yang, F.-Y., & Huang, S.-F. (2016). Behavioural Assessment of Blood-Brain Barrier Opening Induced by Various Ultrasound Parameters. Proceedings of Engineering and Technology Innovation, 3, 13-15. Retrieved from