A Designed Enclosure Unit for Loop-Mediated Isothermal Amplification

Authors

  • Arayan Wongniyom Jyh Jian Chen Department of Biomechatronics Engineering, National Pingtung University of Science and Technology, Pingtung, Taiwan, ROC. Tel. 886-8-7703202 Email: chaucer@mail.npust.edu.tw
  • Jyh-Jian Chen Jyh Jian Chen Department of Biomechatronics Engineering, National Pingtung University of Science and Technology, Pingtung, Taiwan, ROC. Tel. 886-8-7703202 Email: chaucer@mail.npust.edu.tw

Keywords:

phase change materials, LAMP, paraffin wax

Abstract

The gene amplification technique is used in medical science and biotechnology popularly. The loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method that can be utilized in low-resource settings and does not require expensive or complex instruments. The annealing process of LAMP is performed at a constant temperature (around 65 °C) for an hour. From previous works, phase change materials (PCMs) have been applied for the latent heat energy storages due to their isothermal operation during phase transitions. The utilization of the melting temperature of the paraffin wax to maintain the suitable temperature for LAMP process is introduced in our study. To enhance the heat transfer inside the energy storage unit, several pin fins are constructed on the inner surface of the unit which is 50 mm × 50 mm × 50 mm in inner size. The influence of various numbers of pin fins on the temperature distribution is examined by the numerical simulation. The heat transfer inside the paraffin enclosure with fins is better than that without fin. It can be found that the temperature uniformity in the PCM inside the enclosure with fins shows much better. Though the numbers of the fin are increased, some transfer energy from the heat source remains at the enclosure wall and cannot penetrate into the PCM. The duration for all PCM melted from solid phase to liquid phase is over 10 minutes. In the future, the geometric parameters on the duration for phase transition of PCM inside the LAMP chamber will be investigated.

References

E. Aryan, M. Makvandi, A. Farajzadeh, K. Huygen, P. Bifani, and S. L. Mousavi et al, “A novel and more sensitive loop-mediated isothermal amplification assay targeting IS6110 for detection of Mycobacterium tuberculosis complex,” Microbiological Research, vol. 165, no. 3, pp. 211-220, 2010.

Y. Mori and T. Notomi, “Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases,” Journal of Infection and Chemotherapy, vol. 15, no. 2, pp. 62-69, 2009

T. Iwamoto, T. Sonobe, and K. Hayashi, “Loop-mediated isothermal amplification for direct detection of mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples,” Journal of Clinical Microbiology, vol. 41, no. 6, pp. 2616-2622, 2003.

T. Notomi, H. Okayama, H. Masubuchi, T. Yonekawa, K. Watanabe, and N. Amino et al, “Loop-mediated isothermal amplification of DNA,” Nucleic Acids Research, vol. 28, no. 12, pp. e63-e63, 2000.

C. C. Boehme, P. Nabeta, G. Henostroza, R. Raqib, Z. Rahim, and M. Gerhardt et al., “Operational feasibility of using loop-mediated isothermal amplification for diagnosis of pulmonary tuberculosis in microscopy centers of developing countries,” Journal of Clinical Microbiology, vol. 45 no. 6, pp. 1936-1940, 2007.

C. Li, J. Chen, H. Shi, X. Zhang, D. Shi, and X. Han et al., “Rapid detection of porcine kobuvirus in feces by reverse transcription loop-mediated isothermal amplification,” Virology Journal, vol. 11, no. 1, pp. 73, 2014.

H. Mehling and L. F. Cabeza, Heat and cold storage with PCM, Berlin, Springer, 2008.

M. K. Rathod and J. Banerjee, “Thermal stability of phase change materials used in latent heat energy storage systems: a review,” Renewable and Sustainable Energy Reviews, vol. 18, pp. 246-258, 2013.

M. Akgün, O. Aydın, and K. Kaygusuz, “Experimental study on melting/solidification characteristics of a paraffin as PCM,” Energy Conversion and Management, vol. 48, no. 2, pp. 669-678, 2007.

R. Kandasamy, X. Q. Wang and A. S. Mujumdar, “Transient cooling of electronics using phase change material (PCM)-based heat sinks,” Applied Thermal Engineering, vol. 28, no. 8, pp. 1047-1057, 2008.

K. C. Nayak, S. K. Saha, K. Srinivasan, and P. Dutta, “A numerical model for heat sinks with phase change materials and thermal conductivity enhancers,” International Journal of Heat and Mass Transfer, vol. 49, no. 11, pp. 1833-1844, 2006.

R. Pakrouh, M. J. Hosseini, A. A. Ranjbar, and R. Bahrampoury, “A numerical method for PCM-based pin fin heat sinks optimization,” Energy Conversion and Management, vol. 103, pp. 542-552, 2015.

S. F. Hosseinizadeh, F. L. Tan, and S. M. Moosania, “Experimental and numerical studies on performance of PCM-based heat sink with different configurations of internal fins,” Applied Thermal Engineering, vol. 31, no. 17, pp. 3827-3838, 2011.

B. Kamkari and H. Shokouhmand, “Experimental investigation of phase change material melting in rectangular enclosures with horizontal partial fins,” International Journal of Heat and Mass Transfer, vol. 78, pp. 839-851, 2014.

K. A. Curtis, D. L. Rudolph, I. Nejad, J. Singleton, A. Beddoe, and B. Weigl et al., “Isothermal amplification using a chemical heating device for point-of-care detection of HIV-1,” PloS One, vol. 7, no. 2, p. e31432, 2012.

J. Singleton, J. L. Osborn, L. Lillis, K. Hawkins, D. Guelig, and W. Price et al., “Electricity-free amplification and detection for molecular point-of-care diagnosis of HIV-1,” PLoS One, vol. 9, no. 11, p. e113693, 2014.

J. Singleton, C. Zentner, J. Buser, P. Yager, P. LaBarre, and B. H. Weigl, “Instrument-free exothermic heating with phase change temperature control for paper microfluidic devices,” SPIE Proceedings, vol. 8615, p. 86150R, 2013.

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Published

2017-12-20

How to Cite

[1]
A. Wongniyom and J.-J. Chen, “A Designed Enclosure Unit for Loop-Mediated Isothermal Amplification”, Proc. eng. technol. innov., vol. 7, pp. 01–07, Dec. 2017.

Issue

Vol. 7 (2017)

Section

Articles

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