Effect of Organic Loading Rates on Performance of Treating Dairy Wastewater in a Lab-Scale Sequencing Batch Reactor

  • Khac-Uan Do School of Environmental Science and Technology, Hanoi University of Science and Technology, Hanoi, Vietnam
  • Thuy-Ngan Thi Bui Center for Advanced Materials Technology, National Center for Technological Progress, Hanoi, Vietnam
  • Hung-Thuan Tran Center for Advanced Materials Technology, National Center for Technological Progress, Hanoi, Vietnam
  • Xuan-Quang Chu Center for Advanced Materials Technology, National Center for Technological Progress, Hanoi, Vietnam
Keywords: dairy wastewater treatment, sequencing batch reactor, organic loading rates, sludge


This study aims to investigate, the effect of organic loading rates (OLRs), nutrient ratio addition, and sludge retention time (SRT) on treating dairy wastewater in a sequencing batch reactor (SBR) system. This investigation is verified by experiments conducted in 3 phases at 3 different OLRs (1.8, 1.2, and 0.9 kg/m3d, respectively).  Urea ((NH2)2CO) is added to make a suitable (COD:N:P) ratio of (100:5:1) in dairy wastewater. The SRT is adjusted from 50 days to an appropriate value of 18 days. The obtained results show that the COD, TN, and TP removal efficiencies are increased with decreasing OLRs. Sludge concentration in the SBR tank is stable at 1100 mg/L after adding (NH2)2CO. In addition, the SBR operated at a suitable SRT (i.e. 18 days) helps the biomass stably, resulting in enhancement of COD, TN, and TP removal. The results are helpful to the design of SBR for treating dairy wastewater.


Y. Shao, G. H. Liu, Y. Wang, Y. Zhang, H. Wang, L. Qi, et al., “Sludge Characteristics, System Performance and Microbial Kinetics of Ultra-Short-SRT Activated Sludge Processes,” Environment International, vol. 143, article no. 105973, October 2020.

H. Fernandes, M. K. Jungles, H. Hoffmann, R. V. Antonio, and R. H. R. Costa, “Full-Scale Sequencing Batch Reactor (SBR) for Domestic Wastewater: Performance and Diversity of Microbial Communities,” Bioresource Technology, vol. 132, pp. 262-268, March 2013.

A. S. Stasinakis, P. Charalambous, and I. Vyrides, “Dairy Wastewater Management in EU: Produced Amounts, Existing Legislation, Applied Treatment Processes and Future Challenges,” Journal of Environmental Management, vol. 303, article no. 114152, February 2022.

R. Verma, S. Suthar, N. Chand, and P. K. Mutiyar, “Phycoremediation of Milk Processing Wastewater and Lipid-Rich Biomass Production Using Chlorella Vulgaris under Continuous Batch System,” Science of the Total Environment, vol. 833, article no. 155110, August 2022.

M. H. Trevor Sparks, “Water Treatment: Combined Treatment Systems Meet Vietnamese Challenge,” Filtration + Separation, vol. 50, no. 4, pp. 44-45, July-August 2013.

M. Ruhela, A. A. Wani, and F. Ahamad, “Efficiency of Sequential Batch Reactor (SBR) Based Sewage Treatment Plant and Its Discharge Impact on Dal Lake, Jammu & Kashmir, India,” Archives of Agriculture and Environmental Science, vol. 5, no. 4, pp. 517-524, December 2020.

L. Hao, K. Okano, C. Zhang, Z. Zhang, Z. Lei, C. Feng, et al., “Effects of Levofloxacin Exposure on Sequencing Batch Reactor (SBR) Behavior and Microbial Community Changes,” Science of The Total Environment, vol. 672, pp. 227-238, July 2019.

J. Yao, J. Liu, Y. Zhang, S. Xu, Y. Hong, and Y. Chen, “Adding an Anaerobic Step Can Rapidly Inhibit Sludge Bulking in SBR Reactor,” Scientific Reports, vol. 9, article no. 10843, July 2019.

C. Estrada-Vázquez, A. Salinas-Pacheco, E. Peralta-Reyes, H. M. Poggi-Varaldo, and A. Regalado-Méndez, “Parametric Optimization of Domestic Wastewater Treatment in an Activated Sludge Sequencing Batch Reactor Using Response Surface Methodology,” Journal of Environmental Science and Health, Part A, vol. 54, no. 12, pp. 1197-1205, 2019.

J. Liu, J. Li, S. Piché-Choquette, and B. Sellamuthu, “Roles of Bacterial and Epistylis Populations in Aerobic Granular SBRs Treating Domestic and Synthetic Wastewaters,” Chemical Engineering Journal, vol. 351, pp. 952-958, November 2018.

U. Showkat and I. A. Najar, “Study on the Efficiency of Sequential Batch Reactor (SBR)-Based Sewage Treatment Plant,” Applied Water Science, vol. 9, no. 1, article no. 2, February 2019.

L. Pelaz, A. Gómez, A. Letona, G. Garralón, and M. Fdz-Polanco, “Sequencing Batch Reactor Process for the Removal of Nitrogen from Anaerobically Treated Domestic Wastewater,” Water Science & Technology, vol. 77, no. 6, pp. 1581-1590, March 2018.

C. Wu, Y. Peng, S. Wang, X. Li, and R. Wang, “Effect of Sludge Retention Time on Nitrite Accumulation in Real-Time Control Biological Nitrogen Removal Sequencing Batch Reactor,” Chinese Journal of Chemical Engineering, vol. 19, no. 3, pp. 512-517, June 2011.

A. H. Khalaf, W. A. Ibrahim, M. Fayed, and M. G. Eloffy, “Comparison between the Performance of Activated Sludge and Sequence Batch Reactor Systems for Dairy Wastewater Treatment under Different Operating Conditions,” Alexandria Engineering Journal, vol. 60, no. 1, pp. 1433-1445, February 2021.

M. Custodio, R. Peñaloza, C. Espinoza, W. Espinoza, and J. Mezarina, “Treatment of Dairy Industry Wastewater Using Bacterial Biomass Isolated from Eutrophic Lake Sediments for the Production of Agricultural Water,” Bioresource Technology Reports, vol. 17, article no. 100891, February 2022.

R. B. Baird, A. D. Eaton, and E. W. Rice, Standard Methods for the Examination of Water and Wastewater, 23rd ed., Washington: the American Public Health Association (APHA), the American Water Works Association (AWWA), and the Water Environment Federation (WEF), 2017.

J. Fan, F. Ji, X. Xu, Y. Wang, D. Yan, X. Xu, et al., “Prediction of the Effect of Fine Grit on the MLVSS/MLSS Ratio of Activated Sludge,” Bioresource Technology, vol. 190, pp. 51-56, August 2015.

O. Alagha, A. Allazem, A. A. Bukhari, I. Anil, and N. D. Muazu, “Suitability of SBR for Wastewater Treatment and Reuse: Pilot-Scale Reactor Operated in Different Anoxic Conditions,” International Journal of Environmental Research and Public Health, vol. 17, no. 5, article no. 1617, March 2020.

Y. Yuan, J. Liu, B. Ma, Y. Liu, B. Wang, and Y. Peng, “Improving Municipal Wastewater Nitrogen and Phosphorous Removal by Feeding Sludge Fermentation Products to Sequencing Batch Reactor (SBR),” Bioresource Technology, vol. 222, pp. 326-334, December 2016.

M. Vukovic, F. Briški, M. Matošic, and I. Mijatovic, “Analysis of the Activated Sludge Process in an MBR under Starvation Conditions,” Chemical Engineering & Technology, vol. 29, no. 3, pp. 357-363, March 2006.

G. Vijayan, R. Saravanane, and T. Sundararajan, “Influence of Nitrogen Residue on the Performance of Sequencing Batch Reactor (SBR) in Wastewater Treatment–A Review,” International Journal of Environmental Monitoring and Protection, vol. 3, no. 1, pp. 1-16, February 2016.

Y. Duan, Y. Liu, M. Zhang, Y. Li, W. Zhu, M. Hao, et al., “Start-Up and Operational Performance of the Partial Nitrification Process in a Sequencing Batch Reactor (SBR) Coupled with a Micro-Aeration System,” Bioresource Technology, vol. 296, article no. 122311, January 2020.

S. Basu and B. M. Kumar, “Evaluation of Bench Scale SBR for Removal of Nutrients from Domestic Wastewater,” World Environmental and Water Resources Congress, May 2006. https://doi.org/10.1061/40856(200)16

B. Gil-Pulido, E. Tarpey, E. L. Almeida, W. Finnegan, X. Zhan, A. D. W. Dobson, et al., “Evaluation of Dairy Processing Wastewater Biotreatment in an IASBR System: Aeration Rate Impacts on Performance and Microbial Ecology,” Biotechnology Reports, vol. 19, article no. e00263, September 2018.

M. Abdulgader, Q. J. Yu, A. Zinatizadeh, and P. Williams, “Biological Treatment of Milk Processing Wastewater in a Sequencing Batch Flexible Fibre Biofilm Reactor,” Asia-Pacific Journal of Chemical Engineering, vol. 4, no. 5, pp. 698-703, September/October 2009.

S. Sirianuntapiboon, N. Jeeyachok, and R. Larplai, “Sequencing Batch Reactor Biofilm System for Treatment of Milk Industry Wastewater,” Journal of Environmental Management, vol. 76, no. 2, pp. 177-183, July 2005.

J. P. Kushwaha, V. C. Srivastava, and I. D. Mall, “Sequential Batch Reactor for Dairy Wastewater Treatment: Parametric Optimization; Kinetics and Waste Sludge Disposal,” Journal of Environmental Chemical Engineering, vol. 1, no. 4, pp. 1036-1043, December 2013.

K. Kim, “The Characteristic of the Sequencing Batch Reactor (SBR), Anaerobic Sequencing Batch Reactor (ASBR) and Sequencing Batch Biofilm Reactor (SBBR),” https://home.engineering.iastate.edu/~tge/ce421-521/koobum.pdf, June 08, 2022.

M. Kraume, R. Scheumann, A. Baban, and B. El Hamouri, “Performance of a Compact Submerged Membrane Sequencing Batch Reactor (SM-SBR) for Greywater Treatment,” Desalination, vol. 250, no. 3, pp. 1011-1013, January 2010.

C. Tayà, A. Guisasola, and J. A. Baeza, “Assessment of a Bioaugmentation Strategy with Polyphosphate Accumulating Organisms in a Nitrification/Denitrification Sequencing Batch Reactor,” Bioresource Technology, vol. 102, no. 17, pp. 7678-7684, September 2011.

How to Cite
Khac-Uan Do, Thuy-Ngan Thi Bui, Hung-Thuan Tran, and Xuan-Quang Chu, “Effect of Organic Loading Rates on Performance of Treating Dairy Wastewater in a Lab-Scale Sequencing Batch Reactor”, Int. j. eng. technol. innov., Jan. 2023.