FTIR Analysis of Biodegradation of Polystyrene by Intestinal Bacteria Isolated from Zophobas Morio and Tenebrio Molitor

Authors

  • Hong-Han Lin Department of Biological Science & Technology, I-Shou University, Kaohsiung, Taiwan
  • Hsiao-Han Liu Department of Biological Science & Technology, I-Shou University, Kaohsiung, Taiwan

DOI:

https://doi.org/10.46604/peti.2021.5450

Keywords:

polystyrene, tenebrio molitor, zophobas morio, FTIR

Abstract

Since the 1970s, polystyrene (PS) plastic marine pollution has become a global concern. Tenebrio molitor and Zophobas morio beetle larva actively respond to a diet of primarily polystyrene. The tantalizing evidence indicates that the gut bacteria of these beetle larva enables them not only to consume polystyrene, but to successfully biodegrade polystyrene. Heretofore, data collection to verify polystyrene degradation by the gut bacteria of these larva has taken up to six months per test. Our laboratory created a platform by dissolving PS into a liquid mineral culture medium to examine PS degradation by the gut bacteria. Under investigation, PS works as main carbon source supporting the growth of gut bacteria. Fourier-transform Infrared spectroscopy (FTIR) is an effective method which can provide relevant information on chemical changes. This study presents a methodology using FTIR and Visible Light Spectrometry as an efficient methodology to verify the physiological degradation of polystyrene.

References

S. S. Yang, A. M. Brandon, D. F. Xing, J. Yang, J. W. Pang, C. S. Criddle, et al., “Progresses in Polystyrene Biodegradation and Prospects for Solutions to Plastic Waste Pollution,” IOP Conference Series: Earth and Environmental Science, vol. 150, pp. 1-10, May 2018.

R. E. Schnurr, V. Alboiu, M. Chaudhary, R. A. Corbett, M. E. Quanz, K. Sankar, et al., “Reducing marine Pollution from Single-Use Alastics (SUPs): A Review,” Marine Pollution Bulletin, vol. 137, pp. 157-171, December 2018.

L. Yin, H. Liu, H. Cui, B. Chen, L. Li, and F. Wu, “Impacts of Polystyrene Microplastics on the Behavior and Metabolism in a Marine Demersal Teleost, Black Rockfish (Sebastes Schlegliei),” Journal of Hazardous Materials, vol. 380, pp. 120861, December 2019.

M. Revel, A. Châtel, and C. Mouneyrac, “Micro (nano) Plastics: A Threat to Human Health?” Current Opinion in Environmental Science & Health, vol. 1, pp. 17-23, February 2018.

Z. L. Tang, T. A. Kuo, and H. H. Liu, “The Study of the Microbes Degraded Polystyrene,” Advanced Technology Innovation, vol. 2, pp. 13-17, 2017.

B. Stuart, “FTIR of Biomolecules,” Reviews in Cell Biology and Molecular Medicine, https://onlinelibrary.wiley.com/doi/abs/10.1002/3527600906.mcb.200300059, 2006.

S. Bonhomme, A. Cuer, A. Delort, J. Lemaire, M. Sancelme, and G. Scott, “Environmental Biodegradation of Polyethylene,” Polymer Degradation and Stability, vol. 81, no. 3, pp. 441-452, 2003.

G. Chen, S. Liu, S. Chen, and Z. Qi, “FTIR Spectra, Thermal Properties, and Dispersibility of a Polystyrene/Montmorillonite Nanocomposite,” Macromolecular Chemistry and Physics, vol. 202, no. 7, pp. 1189-1193, April 2001.

D. Olmos, E. Martin, and J. Gonzalez-Benito, “New Molecular-Scale Information on Polystyrene Dynamics in PS and PS–BaTiO 3 Composites from FTIR Spectroscopy,” Physical Chemistry Chemical Physics, vol. 16, no. 44, pp. 24339-24349, 2014.

H. Kundungal, M. Gangarapu, S. Sarangapani, A. Patchaiyappan, and S. P. Devipriya, “Efficient Biodegradation of Polyethylene (HDPE) Waste by the Plastic-Eating Lesser Waxworm (Achroia Grisella),” Environmental Science and Pollution Research, vol. 26, no. 18, pp. 18509-18519, May 2019.

Yi-Feng Hsieh, “Degradation of Polyethylene Succinate by Thermophilic Microbispora sp. BC35T-4,” Master's dissertation, Department of Life Science, Fu-Jen University, 2008.

H. K. Chan, B. Ongpipattanakul, and J. Au. Yeung, “Aggregation of rhDNase Occurred During the Compression of KBr Pellets Used for FTIR Spectroscopy,” Pharmaceutical Research, vol. 13, no. 2, pp. 238-242, February 1996.

H. Auta, C. Emenike, and S. Fauziah, “Screening of Bacillus Strains Isolated from Mangrove Ecosystems in Peninsular Malaysia for Microplastic Degradation,” Environmental Pollution, vol. 231, pp. 1552-1559, December 2017.

J. Augusta, R. J. Müller, and H. Widdecke, “A Rapid Evaluation Plate-Test for the Biodegradability of Plastics,” Applied Microbiology and Biotechnology, vol. 39, no. 4-5, pp. 673-678, July 1993.

M. Timmins, D. Gilmore, N. Lotti, M. Scandola, R. Fuller, and R. Lenz, “A Spectrophotometric Method for Detection of Enzymatic Degradation of Thin Polymer Films,” Journal of Environmental Polymer Degradation, vol. 5, no. 1, pp. 1-15, 1997.

M. Sobkowiak and P. Painter, “Determination of the Aliphatic and Aromatic CH Contents of Coals by FT-ir: Studies of Coal Extracts,” Fuel, vol. 71, no. 10, pp. 1105-1125, October 1992.

A. N. A. El. Hendawy, “Variation in the FTIR Spectra of a Biomass under Impregnation, Carbonization and Oxidation Conditions,” Journal of Analytical and Applied Pyrolysis, vol. 75, no. 2, pp. 159-166, March 2006.

K. Nakamiya, G. Sakasita, T. Ooi, and S. Kinoshita, “Enzymatic Degradation of Polystyrene by Hydroquinone Peroxidase of Azotobacter Beijerinckii HM121,” Journal of Fermentation and Bioengineering, vol. 84, no. 5, pp. 480-482, 1997.

R. S. Yao, M. Sun, C. L. Wang, and S. S. Deng, “Degradation of Phenolic Compounds with Hydrogen Peroxide Catalyzed by Enzyme from Serratia Marcescens AB 90027,” Water research, vol. 40, no. 16, pp. 3091-3098, September 2006.

M. D. Faber, “Microbial Degradation of Recalcitrant Compounds and Synthetic Aromatic Polymers,” Enzyme and Microbial Technology, vol. 1, no. 4, pp. 226-232, October 1979.

B. Y. Peng, Y. Su, Z. Chen, J. Chen, X. Zhou, M. E. Benbow, et al., “Biodegradation of Polystyrene by Dark (Tenebrio Obscurus) and Yellow (Tenebrio Molitor) Mealworms (Coleoptera: Tenebrionidae),” Environmental Science & Technology, vol. 53, no. 9, pp. 5256-5265, April 2019.

Y. Yang, J. Wang, and M. Xia, “Biodegradation and Mineralization of Polystyrene by Plastic-Eating Superworms Zophobas Atratus,” Science of the Total Environment, vol. 708, p. 135233, March 2020.

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Published

2021-01-20

How to Cite

[1]
Hong-Han Lin and H.-H. Liu, “FTIR Analysis of Biodegradation of Polystyrene by Intestinal Bacteria Isolated from Zophobas Morio and Tenebrio Molitor”, Proc. eng. technol. innov., vol. 17, pp. 50–57, Jan. 2021.

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